( 2013).ĭaily energy expenditure and its components ( 2012), Even and Nadkarni ( 2012), Speakman et al. Several other publications contain useful information on similar issues that are directly pertinent to the measurement of energy metabolism in the mouse and the reader may also wish to consult these, in particular the papers by Weir ( 1949), Kleiber ( 1961), Ferrannini ( 1988), Simonson and deFronzo ( 1990), Bursztein et al. I will therefore also draw on some examples in the literature of studies on such animals. These considerations apply more generally to other small mammals in the same size range as mice (i.e., <100 g).
#Chea pmouse species how to#
It also addresses the issue of how to analyze the resulting data and some of the pitfalls in this analysis. This paper concerns theoretical and practical considerations for measuring the energy metabolism of the mouse.
Part of this effort will be to understand the impact that individual genes have on energy metabolism, and their consequences for disorders such as obesity (Speakman et al., 2008 Hall et al., 2012). Ascertaining the functions of the 30,000 or so genes in the human genome will be facilitated enormously by the study of the mouse in the coming decades. This gives us phenomenal capabilities to explore the relationships between individual and multiple genes and their phenotypic consequences.
What sets the mouse apart, however, is the technological capability to manipulate its genome to generate animals with global and tissue specific knock-out and transgenic models. The rat is also a mammalian endotherm that shares much of its genome and physiology with the human. Despite millions of years of evolutionary divergence the mouse has extremely close synteny of its genome with the human (Peltonen and McKusick, 2001), and physiologically, being a mammal and an endotherm, it shares many features of human metabolism not found in the other animal models such as ectothermic invertebrates like Drosophila melanogaster and Caenorhabditis elegans. The mouse is probably the most important species as a model for the study of human diseases and disorders. The statistically most appropriate approach is to use analysis of covariance with individual aspects of body composition as independent predictors. This has been a matter of some discussion for at least 120 years. The greatest analytical challenge for mouse expenditure data is how to account for body size differences between individuals. These latter systems are ideal for measuring total daily energy expenditure but at present do not allow accurate decomposition of the total expenditure into its components. The other type of system involves a large chamber which mimics the home cage environment and is generally configured with several chambers/analyzer. Two types of system are available – one of which involves a single small Spartan chamber linked to a single analyzer, which is ideal for measuring the individual components of energy demand. Energy expenditure in mice is normally measured using open flow indirect calorimetry apparatus. Theoretically total daily energy expenditure is comprised of several different components: basal or resting expenditure, physical activity, thermoregulation, and the thermic effect of food. This paper considers some theoretical, practical, and analytical considerations to be considered when measuring energy expenditure in mice. Measuring energy metabolism in the mouse presents a challenge because the animals are small, and in this respect it presents similar challenges to measuring energy demands in many other species of small mammal. This includes characterization of the factors that influence energy expenditure and dysregulation of energy balance leading to obesity and its sequelae.
The mouse is one of the most important model organisms for understanding human genetic function and disease.
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